Protein Expression Eukaryotic cells T. thermophila

Get tips on using pcDNA-oGP1 to perform Protein Expression Eukaryotic cells - HEK293 GP1

Get tips on using pMT3-RhoGC to perform Protein Expression Eukaryotic cells - HEK293 RhoGC

Get tips on using pONE-40A to perform Protein Expression Eukaryotic cells - HEK293 MBP

Get tips on using PL_Mm Ppp1r15a to perform Protein Expression Eukaryotic cells - CHO Ppp1r15a

Get tips on using PL_Mm P4hb to perform Protein Expression Eukaryotic cells - CHO P4hb

Get tips on using pGS-CD62L to perform Protein Expression Eukaryotic cells - CHO CD62L

Get tips on using pMH3-rFVII to perform Protein Expression Eukaryotic cells - CHO rFVII

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using pBGP3-RsEG to perform Protein Expression Eukaryotic cells - P. pastoris cellulases

Get tips on using pBGP3-NtEG to perform Protein Expression Eukaryotic cells - P. pastoris cellulases