rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

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Get tips on using CelLytic™ NuCLEAR™ Extraction Kit to perform Protein isolation Mammalian cells - BHK-21

Products Sigma-Aldrich CelLytic™ NuCLEAR™ Extraction Kit

Get tips on using StemSep™ Human CD34 Positive Selection Cocktail to perform Cell Isolation CD34+ cells

Products STEMCELL technologies StemSep™ Human CD34 Positive Selection Cocktail

Get tips on using MACSprep™ Chimerism CD34 MicroBead Kit, human to perform Cell Isolation CD34+ cells

Products Miltenyibiotec MACSprep™ Chimerism CD34 MicroBead Kit, human

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - SK-N-BE(2)-C

Products Sigma-Aldrich RIPA Buffer

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

DNA CRISPR Mouse Deletion ES (embryonic stem) cells Etv2 promoter

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - MLS-1765

Products Thermo Fisher Scientific RIPA Lysis and Extraction Buffer

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - BHK-21

Products Thermo Fisher Scientific RIPA Lysis and Extraction Buffer

Get tips on using Expi293™ Expression System Kit to perform Protein expression and purification Mammalian cells - HEK 293 Mt-PA

Products Thermo Fisher Scientific Expi293™ Expression System Kit

Get tips on using ExpiCHO™ Expression System Kit to perform Protein expression and purification Mammalian cells - CHO-K1 SUMO protein

Products Thermo Fisher Scientific ExpiCHO™ Expression System Kit

Get tips on using pcDNA™3.1 (+) Mammalian Expression Vector to perform Protein expression and purification Mammalian cells - CHO-K1 sRAGE

Products Thermo Fisher Scientific pcDNA™3.1 (+) Mammalian Expression Vector

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