Site Directed Mutagenesis (SDM) Human Point mutation BxPC-3

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells to perform Reporter gene assay β-galactosidase substrates - MCF-7 human breast cancer

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Get tips on using ON-TARGETplus Rat Rhog (308875) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 RhoG

Get tips on using ON-TARGETplus Rat Trio (310192) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Trio

Get tips on using ON-TARGETplus Rat Dcp1a (361109) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - PC12 Dcp1a

Get tips on using ON-TARGETplus Rat Lsm1 (364624) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - PC12 Lsm1

Get tips on using ON-TARGETplus Rat Atg7 (312647) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - UMR‐106 Atg7

Get tips on using ON-TARGETplus Rat Arhgef12 (367072) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Larg/Arhgef12