siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using pAC2-dual-dCas9VP48-sgExpression to perform CRISPR Human - Activation IL1RN

Get tips on using UltraCruz® Transfection Reagent to perform CRISPR Human - Activation GRP78

Get tips on using pAC2-dual-dCas9VP48-sgExpression to perform CRISPR Human - Activation SOX2

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Notch1 (C44H11) Rabbit mAb #3268 to perform Immunohistochemistry Human - Notch1

Get tips on using Anti-p53 Protein, Clone DO7 to perform Immunohistochemistry Human - p53

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - RCC

Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Human - OSCC

Get tips on using TruSeq ChIP Library Preparation Kit to perform ChIP Human - PBMC

Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Human - LCL