RNA isolation / purification Cells Cancer cell lines

Get tips on using TruSeq Small RNA Library Preparation Kit to perform RNA sequencing Human - HEK293T

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - HEK293T

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - HeLa

Get tips on using Live/Dead cell Staining Kit II to perform Live / Dead assay mammalian cells - mouse iPSC

Get tips on using Live/Dead cell Staining Kit II to perform Live / Dead assay mammalian cells - NIH/3T3

Get tips on using Live-Dead Cell Staining Kit (BioVision) to perform Live / Dead assay mammalian cells - NIH/3T3

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - mouse keratinocytes

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - SK-N-BE(2)-C

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.