Site Directed Mutagenesis (SDM) Dog Insertion

Get tips on using EZ1 DNA Tissue Kit (48) to perform DNA isolation / purification Tissue - FFPE samples

Get tips on using MagAttract PowerSoil DNA KF Kit to perform DNA isolation / purification Tissue - fecal sample

Get tips on using MagAttract PowerMicrobiome DNA/RNA Kit to perform DNA isolation / purification Micorbiome - Fecal sample

Get tips on using MagAttract PowerSoil DNA EP Kit to perform DNA isolation / purification Micorbiome - Fecal sample

Get tips on using miScript PreAMP PCR Kit (60) to perform PCR Preamplification of cDNA - FFPE samples

Get tips on using pMHL_2002-bfloGFPa1 to perform Protein Expression Eukaryotic cells - T. pseudonana IbpA DR2 antigen from Histophilus somni

Get tips on using pMSCV-LTR-dCas9-VP64-BFP to perform CRISPR Mouse - Activation Neuro-2a Sim1

Get tips on using AllPrep DNA/RNA Mini Kit to perform DNA isolation / purification Tissue - fecal sample

The challenge in isolating RNA from S. aureus cells is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads is considered to be the better alternative.

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads could be the best alternative.