siRNA / RNAi /miRNA transfection Human Cells THP-1

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - mouse liver tissue

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - mouse muscle tissue

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Bacteria - Gram positive Listeria monocytogens

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Bacteria - Gram negative Bordetella pertussis

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

Get tips on using Gentra Puregene Blood Kit to perform DNA isolation / purification Cells - Primary cells Bone marrow mononuclear cells

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Get tips on using RNAprep pure Kit to perform RNA isolation / purification Tissue - Human Placenta

Get tips on using HES1 (D6P2U) Rabbit mAb #11988 to perform Immunohistochemistry Human - Hes1

Get tips on using 1ml NCL-L-CK20-561 to perform Immunohistochemistry Human - CK20