siRNA / RNAi /miRNA transfection Rat Ar4-2j (Pancreatic tumor)

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A549

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using pSilencer™ 4.1-CMV neo to perform shRNA gene silencing Human - SiHa AEG-1

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized A549

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized A549

Get tips on using pSilencer 2.1-U6 Hygro to perform shRNA gene silencing Human - Neuroblastoma cells (SH-SY5Y) Beclin 1

Get tips on using AM5770: pSilencer™ 3.1-H1 neo to perform shRNA gene silencing Mouse - CT26 OPN

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized A549