Site Directed Mutagenesis (SDM) Human Deletion HEK 293T

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Get tips on using pJAP2 to perform Protein Expression Eukaryotic cells - HEK293 AT1R

Get tips on using pJAP34 to perform Protein Expression Eukaryotic cells - HEK293 A1R

Get tips on using pJAP37 to perform Protein Expression Eukaryotic cells - HEK293 A1R

Get tips on using YFP‐TOP2β to perform Protein Expression Eukaryotic cells - HEK293 TOP2β

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Get tips on using pcDNA-nGP1 to perform Protein Expression Eukaryotic cells - HEK293 GP1

Get tips on using pcDNA-oGP1 to perform Protein Expression Eukaryotic cells - HEK293 GP1

Get tips on using pMT3-RhoGC to perform Protein Expression Eukaryotic cells - HEK293 RhoGC

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