Get tips on using pgMAX system-Bgeo to perform Protein Expression Prokaryotic cells - E. coli Bgeo
Get tips on using pHT43-BMP2-D to perform Protein Expression Prokaryotic cells - B. subtilis rhBMP2
Get tips on using pHT43-BMP2-M to perform Protein Expression Prokaryotic cells - B. subtilis rhBMP2
Get tips on using pJL TRBO-G to perform Protein Expression Prokaryotic cells - A. tumefaciens GFP
A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.
A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.
Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - KC02-44D
Get tips on using pFastBac1-sccaIL-12 to perform Protein Expression Eukaryotic cells - Hi5 IL12 canine
Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - THP-1
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