siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

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Get tips on using pgMAX system-Bgeo to perform Protein Expression Prokaryotic cells - E. coli Bgeo

Products Manabu Murakami, Department of Pharmacology, Hirosaki University pgMAX system-Bgeo

Get tips on using pHT43-BMP2-D to perform Protein Expression Prokaryotic cells - B. subtilis rhBMP2

Products Roquyya Gul, Institute of Molecular Biology and Biotechnology/Ce pHT43-BMP2-D

Get tips on using pHT43-BMP2-M to perform Protein Expression Prokaryotic cells - B. subtilis rhBMP2

Products Roquyya Gul, Institute of Molecular Biology and Biotechnology/Ce pHT43-BMP2-M
pJL TRBO-G Product

Get tips on using pJL TRBO-G to perform Protein Expression Prokaryotic cells - A. tumefaciens GFP

Products Sheng Wang, Key Laboratory of Ministry of Education for Protecti pJL TRBO-G

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

DNA DNA gel extraction / PCR product purification Product size < 15Kb

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

DNA DNA gel extraction / PCR product purification Product size > 15Kb

Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - KC02-44D

Products Cell Signaling Technology Cell Lysis Buffer (10X)

Proteins Immunohistochemistry Human GR/glucocorticoid receptor

Get tips on using pFastBac1-sccaIL-12 to perform Protein Expression Eukaryotic cells - Hi5 IL12 canine

Products Geraldo Gileno de Sá Oliveira, Centro de Pesquisas Gonçalo Mon pFastBac1-sccaIL-12

Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - THP-1

Products Merck Millipore EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit

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