CRISPR Mouse Deletion ES (embryonic stem) cells

Get tips on using LC3B monoclonal antibody (2G6) to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using ATG5 Antibody (N-term) to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Atg12 (D88H11) Rabbit mAb to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Gibco™Neurobasal™ Medium to perform 3D Cell Culture Media Mouse embryonic neurospheres

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Mouse embryonic fibroblasts

Get tips on using Mesenchymal Stem Cell Chondrogenic Differentiation Medium to perform Stem cell Differentiation media hBMSCs differentiation into chondrogenic cells

Get tips on using Mesenchymal Stem Cell Chondrogenic Differentiation Medium to perform Stem cell Differentiation media hUMSCs differentiation into chondrogenic cells

Get tips on using Mesenchymal Stem Cell Adipogenic Differentiation Medium to perform Stem cell Differentiation media hUMSCs differentiation into adipogenic cells

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.