Site Directed Mutagenesis (SDM) Human Deletion HepG2

Get tips on using Anti-mTOR antibody to perform Autophagy assay cell type - HepG2

Get tips on using Annexin V-FITC Apoptosis Detection Kit to perform Apoptosis assay cell type - SMMC-7721, HEPG2

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - HepG2

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized HepG2

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized HepG2

Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - HepG2

Get tips on using PI3 Kinase p85 Antibody to perform Autophagy assay cell type - HepG2

Get tips on using Anti-mTOR antibody [53E11] to perform Autophagy assay cell type - HepG2

Get tips on using ATG5 antibody [EPR1755(2)] to perform Autophagy assay cell type - HepG2