Site Directed Mutagenesis (SDM) Human Deletion SKOV3

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using hCas9 to perform CRISPR Mouse - Deletion Dck

Get tips on using hCas9 to perform CRISPR Mouse - Deletion C2C12 Dmd

Get tips on using hCas9 to perform CRISPR Mouse - Deletion B117P Dck

Get tips on using pCas9_GFP to perform CRISPR Mouse - Deletion ANS4 Rosa26

Get tips on using JDS246 to perform CRISPR Mouse - Deletion C2C12 Prnp

Get tips on using MLM3636 to perform CRISPR Mouse - Deletion Neuro 2a Prnp

Get tips on using JDS246 to perform CRISPR Mouse - Deletion Neuro 2a Prnp

Get tips on using lentiCRISPR to perform CRISPR Mouse - Deletion RAW 264.7 Tfeb

Get tips on using hCas9 to perform CRISPR Mouse - Deletion 3T3-L1 ATP7A