Get tips on using Recombinant Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) to perform Western blotting Ubiquitin
Get tips on using ScriptSeq Complete Gold Kit (Epidemiology) to perform RNA sequencing Human - SH-SY5Y
Get tips on using Recombinant Anti-MUC1 antibody [EPR1023] (ab109185) to perform Immunohistochemistry Human - Muc-1
Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Whole genome profiling - mouse liver tissue
Get tips on using QIAshredder (250) to perform DNA fragmentation Cell lines
DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.
Get tips on using Rabbit monoclonal [EP1890Y] to ATM (phospho S1981) to perform Immunohistochemistry ATM phospho - Rabbit IgG Human -NA-
Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.
Get tips on using Recombinant Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) to perform Flow cytometry Anti-bodies Human - Vimentin
Get tips on using cDNA-PCR Sequencing Kit to perform cDNA synthesis Cell lines
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