DNA isolation / purification Cells Primary cells

Get tips on using pIRES2-EGFP-PBD-1 to perform Protein Expression Prokaryotic cells - E. coli PBD1-EGFP

Get tips on using pRSET A-FhFtn-1 to perform Protein Expression Prokaryotic cells - E. coli FhFtn-1

Get tips on using pTRAkc-AH/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp

Get tips on using pTRAkc-ERH/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp

Get tips on using SQSTM1/p62 Antibody to perform Autophagy assay cell type - K562 cells

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using pONE-30A to perform Protein Expression Eukaryotic cells - S. frugiperda MBP

Get tips on using AcP(−)p6.9hSEAP to perform Protein Expression Eukaryotic cells - S. frugiperda hSEAP

Get tips on using AcP(−)p6.9hEPO to perform Protein Expression Eukaryotic cells - S. frugiperda hEPO

Get tips on using pDDGFP-2 to perform Protein Expression Eukaryotic cells - S. cerevisiae GPCRs