RNA isolation / purification Tissue Human

Get tips on using miScript II RT Kit (50) to perform cDNA synthesis Tissue

Get tips on using First-Strand cDNA Synthesis Kit to perform cDNA synthesis Tissue

Get tips on using SuperScript® II Reverse Transcriptase to perform cDNA synthesis Tissue

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using LC3B Antibody to perform Autophagy assay cell type - Rat cerebral coritcal tissue

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Brain tissue

Get tips on using ImProm-II™ Reverse Transcription System to perform cDNA synthesis Tissue

Get tips on using Transcriptor High Fidelity cDNA Synthesis Kit to perform cDNA synthesis Tissue

Get tips on using Anti-Atg5 to perform Autophagy assay cell type - Mouse white adipose tissue