siRNA / miRNA gene silencing Rat INS-1

Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Cells - immortalized human pancreatic cancer

Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Cells - primary human pancreatic stellate cells

Get tips on using DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #14793 to perform ChIP Anti-bodies FLAG

Get tips on using GenLadder 100 bp + 1.5 kbp (ready-to-use), DNA marker to perform DNA Ladder 100 bp

Get tips on using Oligotex mRNA Midi Kit (12) to perform mRNA / Ribonucleoprotein isolation / purification polyA+ mRNA

Get tips on using Oligotex mRNA Mini Kit (12) to perform mRNA / Ribonucleoprotein isolation / purification polyA+ mRNA

Get tips on using MagAttract Direct mRNA M48 Kit (192) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Get tips on using Cytokeratin 19 Antibody to perform Immunohistochemistry Cytokeratin 19 - Rabbit Mouse -NA-

Get tips on using MMP13 Recombinant Rabbit Monoclonal Antibody (3H13L17) to perform Western blotting MMP-13

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.