siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using Anti-panCytokeratin antibody [C-11] (ab7753) to perform Flow cytometry Anti-bodies Human - Keratin

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - adipose stem cells

Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Human - Point mutation U-87MG AQP4

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using GeneArt™ Site-Directed Mutagenesis PLUS System to perform Site Directed Mutagenesis (SDM) Human - Point mutation A498 PIK3CB

Get tips on using Advanced DMEM to perform 3D Cell Culture Media Human cancer esophageal organoids

Get tips on using APC BrdU Flow Kit to perform Cell cycle assay human - OVCAR-5

Get tips on using APC BrdU Flow Kit to perform Cell cycle assay human - HL-60

Get tips on using Pierce™ Magnetic ChIP Kit to perform ChIP Human - Fibroblast cell lines

Get tips on using FxCycle™ Far Red Stain to perform Cell cycle assay human - U20S