Get tips on using Senescence β-Galactosidase Staining Kit - Beyotime to perform Reporter gene assay β-galactosidase substrates - adipose stem cells
Get tips on using AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit to perform Cell line authentication Human iPSC cells derived from human dermal fibroblasts
Get tips on using Silencer® Select GLO-1 siRNA to perform siRNA / miRNA gene silencing Human - Primary Human Aortic Endothelial Cells GLO-1 Lipid
Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary human pulmonary artery endothelial cells
Get tips on using FreeStyle™ 293-F Cells to perform Protein expression and purification Mammalian cells - HEK 293 EGFR
Get tips on using Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide (PI) to perform Apoptosis assay cell type - HeLa cells
Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
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