Get tips on using Anti-Oct4 antibody - ChIP Grade (ab19857) to perform Western blotting Oct4
Get tips on using Anti-Nanog antibody - ChIP Grade (ab21624) to perform Western blotting Nanog
Get tips on using EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit to perform ChIP Human - Glioblastoma cell line
The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.
Get tips on using Recombinant Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063) to perform ChIP Anti-bodies ERα
Get tips on using Anti-Histone H4 (mono methyl K20) antibody - ChIP Grade (ab9051) to perform ChIP Anti-bodies H4K20me1
Get tips on using Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048) to perform ChIP Anti-bodies H3K36me1
Get tips on using Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (ab7766) to perform ChIP Anti-bodies H3K4me2
Get tips on using Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895 to perform ChIP Anti-bodies H3K4me1
Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - ASM
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