Protein Expression Eukaryotic cells P. pastoris

Get tips on using pPIC9K Pichia Vector to perform Protein expression and purification Yeast - Pichia pastoris N-APP

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using pONE-30A to perform Protein Expression Eukaryotic cells - S. frugiperda MBP

Get tips on using AcP(−)p6.9hSEAP to perform Protein Expression Eukaryotic cells - S. frugiperda hSEAP

Get tips on using AcP(−)p6.9hEPO to perform Protein Expression Eukaryotic cells - S. frugiperda hEPO

Get tips on using pDDGFP-2 to perform Protein Expression Eukaryotic cells - S. cerevisiae GPCRs

Get tips on using pFastBac1-sccaIL12opt to perform Protein Expression Eukaryotic cells - Hi5 IL12 canine

Get tips on using pEYK1-3AB to perform Protein Expression Eukaryotic cells - Y. lipolytica CalB

Get tips on using pBI121-MAP30 to perform Protein Expression Eukaryotic cells - N. benthamiana MAP30

Get tips on using pXVE-spAAT to perform Protein Expression Eukaryotic cells - N. benthamiana XVE