Site Directed Mutagenesis (SDM) Human Deletion HEK 293T

Get tips on using GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit to perform CRISPR Human - Deletion DJ-1

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Anti-LC3 pAb to perform Autophagy assay cell type - HEK 293

Get tips on using LC3B Rabbit Antibody to perform Autophagy assay cell type - HEK 293

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - HEK 293

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized HEK 293

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized HEK 293

Get tips on using BECN1 Antibody (H-300) to perform Autophagy assay cell type - HEK 293

Get tips on using Phospho-mTOR (Ser2448) Antibody to perform Autophagy assay cell type - HEK 293