Site Directed Mutagenesis (SDM) Human Point mutation Caco-2

Get tips on using Rat Bone Morphogenetic Protein 2 ELISA to perform ELISA Rat - BMP-2

Get tips on using IEF and 2-D Protein Standards to perform Protein Ladder IEF and 2-D Standards

Get tips on using PCNA Antibody (F-2) to perform Cell cytotoxicity / Proliferation assay cell type - oral squamous cell carcinoma

Get tips on using pwPICZalpha-DT390-bi-pIL-2-Gly to perform Protein Expression Eukaryotic cells - P. pastoris Porcine IL-2 fusion toxins

Get tips on using Rac1/2/3 Antibody #2465 to perform Western blotting Rac1

Get tips on using ErbB2 (HER-2) Antibody Cocktail to perform Western blotting HER2

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using EpiTect 96 Bisulfite Kit (2) to perform Bisulfite DNA Modification Fluids

Get tips on using FaqI (BsmFI) (2 U/µL) to perform Restriction Enzymes BsmFI / FaqI

Get tips on using BspPI (AlwI) (2 U/µL) to perform Restriction Enzymes AlwI / BspPI