PCR

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Cell cytotoxicity / Proliferation assay cell type - K562

Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3

Get tips on using Cultrex® Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3

Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Cardiomyocytes

Get tips on using Renilla luciferase vector, pGL4.74 to perform Reporter gene assay luciferase - primary human endometrial stromal cells

Get tips on using TransIT-TKO Transfection Reagent to perform siRNA / RNAi /miRNA transfection Mouse - Primary Splenocytes Polymer / lipid

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Get tips on using Qproteome FFPE Tissue Kit (20) to perform Protein isolation Tissue - Human tissue C-MFPE samples

Get tips on using pFBDM-PS1(wt)-γ-secretase to perform Protein Expression Eukaryotic cells - S. frugiperda γ-secretase