RNA isolation / purification Bacteria

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Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Human lung fibroblasts (HLF)

RNA siRNA / miRNA gene silencing Human siRNA negative control Lipid

RNA siRNA / miRNA gene silencing Human OV2008 Yap Gene Lipofectamine

RNA siRNA / miRNA gene silencing Human HUVEC IL-8 Lipid

RNA siRNA / miRNA gene silencing Human HeLa EPAS-1 Lipid

RNA siRNA / miRNA gene silencing Mouse siRNA negative control polymer / lipid

RNA siRNA / miRNA gene silencing Mouse Glomerular mesangial cells HIPK2 Polymer / Lipid delivery

RNA siRNA / miRNA gene silencing Human Jurkat MK2 (MAPK Kinase 2) Viral vectors

RNA siRNA / miRNA gene silencing Human U937 MK2 (MAPK Kinase 2) Viral vectors

Get tips on using HiPerFect Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - AR42J Lipid based

Products Qiagen HiPerFect Transfection Reagent

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