Site Directed Mutagenesis (SDM) Dog Point mutation MDCK

Get tips on using SMARTer® PCR cDNA Synthesis Kit to perform cDNA synthesis Yeast

Get tips on using TruSeq Small RNA Library Preparation Kit to perform RNA sequencing Human - HEK293T

Get tips on using Smooth Muscle Cell Growth Medium 2 to perform Mammalian cell culture media HCASMC

Get tips on using Canine Smooth Muscle Cell Growth Medium to perform Mammalian cell culture media CnAOSMC

Get tips on using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® to perform RNA sequencing Mouse - C2C12

Get tips on using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® to perform RNA sequencing Human - HEK293T

Get tips on using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® to perform RNA sequencing Mouse - Neuro 2a

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized DG-75

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized DG-75

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase