siRNA / miRNA gene silencing Mouse B16-BL6

Get tips on using eNOS antibody to perform Western blotting eNOS

Get tips on using SNAI1 antibody to perform Western blotting SNAI1

Get tips on using Oct4 antibody to perform Western blotting Oct4

Get tips on using ABCA1 Antibody to perform Western blotting ABCA1

Get tips on using RAC1 Antibody to perform Western blotting Rac1

Get tips on using GAPDH Antibody to perform Western blotting GAPDH

Get tips on using P53 Antibody to perform Western blotting p53

Get tips on using IKBKB Antibody to perform Western blotting IKK Beta

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.