RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.
Get tips on using FastDigest SdaI to perform Restriction Enzymes SbfI / SdaI
Get tips on using ON-TARGETplus Rat Smad2 (29357) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - IEC-6 Smad2
Get tips on using ON-TARGETplus Rat Smad3 (25631) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - IEC-6 Smad3
Get tips on using Bsp1286I (SduI) restriction enzyme to perform Restriction Enzymes Bsp1286I / SduI
Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?
Get tips on using Mouse SDF1 ELISA Kit (ab100741) to perform ELISA Mouse - SDF-1/CXCL12
Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Mouse small intestinal organoids
Get tips on using IntestiCult™ Organoid Growth Medium (Mouse) to perform 3D Cell Culture Media Mouse small intestinal organoids
Get tips on using Mouse SDF-1 α / CXCL12 α ELISA Kit to perform ELISA Mouse - SDF-1/CXCL12
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment