DNA methylation profiling Gene specific profiling HeLa

Get tips on using EasySep™ HLA B Cell Enrichment Kit to perform Cell Isolation HLA B Cell

Get tips on using EasySep™ HLA T Cell Enrichment Kit to perform Cell Isolation HLA T Cell

Get tips on using RosetteSep™ HLA T Cell Enrichment Cocktail to perform Cell Isolation HLA T Cell

Get tips on using MACSprep™ HLA B Cell Isolation Kit, human to perform Cell Isolation HLA B Cell

Get tips on using MACSprep™ HLA T Cell Isolation Kit, human to perform Cell Isolation HLA T Cell

Get tips on using HLA-DR Monoclonal Antibody (LN3), eBioscience™ to perform Flow cytometry Anti-bodies Human - HLA-DR

Get tips on using MACSprep™ HLA B/T Cell Isolation Kit, human to perform Cell Isolation HLA B/T

Get tips on using Brilliant Violet 510™ anti-human HLA-DR Antibody to perform Flow cytometry Anti-bodies Human - HLA-DR

Get tips on using PerCP-Cy™5.5 Mouse Anti-Human HLA-DR to perform Flow cytometry Anti-bodies Human - HLA-DR

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.