cell-cycle-assay-human-hela

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Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human endometrial stromal cells

Products Qiagen miRNeasy Mini kit

Get tips on using GeneJuice® Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human osteoblasts

Products Millipore GeneJuice® Transfection Reagent

Get tips on using TransIT-TKO Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human astrocytes

Products Mirus TransIT-TKO Transfection Reagent

Get tips on using ZR RNA MiniPrepTM kit to perform RNA isolation / purification Cells - primary human endothelial cells

Products Zymo Research ZR RNA MiniPrepTM kit

Get tips on using mirVana® miRNA mimic to perform RNA isolation / purification Cells - primary human endothelial cells

Products Thermo Fisher Scientific mirVana® miRNA mimic

I work with Human liver organoids and I would like to know the impact of FBS on the organoids. Since the composition of FBS is unknown, would you recommend any alternatives such as human platelet lysate?

Discussions Impact of using FBS

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling mouse hematopoietic stem cells

Get tips on using SPLInsert™ Hanging to perform Cell migration / Invasion cell type - HaCat

Products SPL Life Sciences SPLInsert™ Hanging
Fenozol Product

Get tips on using Fenozol to perform siRNA / miRNA gene silencing Human - BOSC23

Products A&A Biotechnology Fenozol

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression HBV RT

Products Addgene lentiCRISPR v2

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