Immunofluorscence  53BP1 [H-300]

Get tips on using pQE-30 to perform Protein Expression Prokaryotic cells - E. coli Guinea Pig TNF-Alpha

Get tips on using K-Ras Antibody (F234): sc-30 to perform Western blotting Ras

Get tips on using Sall4 Antibody (EE-30): sc-101147 to perform ChIP Anti-bodies Sall4

Get tips on using pQE-30 Xa Vector to perform Protein expression and purification Bacteria - Escherichia coli Siva1

Get tips on using γ Tubulin Antibody (TU-30): sc-51715 to perform Western blotting tubulin gamma

Get tips on using McCoy's 5A Medium, ATCC® 30-2007™ to perform Mammalian cell culture media A253

Get tips on using MycoAlert™ PLUS Mycoplasma Detection Kit (30 Tests) to perform Cell Culture Contamination Detection Kit Mycoplasma

Get tips on using CD45 Monoclonal Antibody (30-F11), PE-Cyanine7, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD45

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.