Site Directed Mutagenesis (SDM) Human Deletion HEK 293T

Get tips on using GABARAP (E1J4E) Rabbit mAb to perform Autophagy assay cell type - HEK 293

Get tips on using Atg16L1 (D6D5) Rabbit mAb to perform Autophagy assay cell type - HEK 293

Get tips on using Notch 1 Antibody (C-20) to perform Autophagy assay cell type - HEK 293

Get tips on using QuantiPro™ BCA Assay Kit to perform Protein quantification Mammalian cells - HEK 293

Get tips on using Zombie Fixable Viability™ Sampler Kit to perform Live / Dead assay mammalian cells - HEK 293

Get tips on using Cell Proliferation ELISA, BrdU to perform Cell cytotoxicity / Proliferation assay cell type - HEK 293

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - HEK 293

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.