Site Directed Mutagenesis (SDM) Human Point mutation Caco-2

Get tips on using TIMP-2 Antibody (B-12): sc-365671 to perform Western blotting TIMP-2

Get tips on using Mouse BMP-2 PicoKine™ ELISA Kit to perform ELISA Mouse - BMP-2

Get tips on using Rat BMP-2 PicoKine™ ELISA Kit to perform ELISA Rat - BMP-2

Get tips on using MAP LC3β Antibody (G-2) to perform Autophagy assay cell type - AR42J

Get tips on using STEMdiff™ APEL™2 Medium to perform 3D Cell Culture Media hiPSC-derived kidney organoids

Get tips on using BLOOD AGAR BASE NO.2 to perform Bacterial cell culture media Helicobacter pylori

Get tips on using Smooth Muscle Cell Growth Medium 2 to perform Mammalian cell culture media HCASMC

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using STEMdiff™ APEL™ 2 Medium to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)