Site Directed Mutagenesis (SDM) Human Point mutation THP-1

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - THP-1

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - THP 1

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - THP 1

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - THP-1

Get tips on using Live/Dead Double Staining Kit (Merck) to perform Live / Dead assay mammalian cells - THP-1

Get tips on using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using RPMI 1640, with L-Glutamine and 25mM HEPES to perform Mammalian cell culture media THP-1

Get tips on using Purified Mouse Anti-Beclin Clone 20/Beclin (RUO) to perform Autophagy assay cell type - THP 1