dna-methylation-profiling-gene-specific-profiling-siha-hpv-16

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.

Get tips on using pSA-HVif-FabV to perform Protein Expression Prokaryotic cells - E. coli HIV-1 vif

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Live/Dead Cell Double Staining Kit to perform Live / Dead assay mammalian cells - HUVEC

Get tips on using Live-Dead Cell Staining Kit (BioVision) to perform Live / Dead assay mammalian cells - HUVEC

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - HUVEC

Get tips on using FastDigest KspAI to perform Restriction Enzymes KspAI / HpaI

Get tips on using FastDigest TaaI to perform Restriction Enzymes TaaI / HpyCH4III

Get tips on using DdeI R6291 to perform Restriction Enzymes DdeI / HpyF3I

Get tips on using FastDigest MspI to perform Restriction Enzymes HpaII / MspI