Protein expression and purification Mammalian cells HEK 293

Get tips on using Vascular Cell Basal Medium to perform Mammalian cell culture media HCAEC

Get tips on using Smooth Muscle Cell Medium to perform Mammalian cell culture media HAOSMC

Get tips on using Canine Endothelial Cell Media to perform Mammalian cell culture media CnAOEC

Get tips on using Bovine Endothelial Cell Media to perform Mammalian cell culture media BAOEC

Get tips on using KAPA LongRange to perform PCR Hot start PCR - Mammalian DNA

Get tips on using PCR SuperMix to perform PCR Conventional / Qualitative PCR - mammalian DNA

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.