Site Directed Mutagenesis (SDM) Monkey Point mutation Cos-7

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004 to perform ChIP Mouse - Panc02

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004 to perform ChIP Mouse - BMDCs

Get tips on using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 to perform ChIP Human - Glioblastoma cell line

Get tips on using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 to perform ChIP Mouse - 3T3-L1 cells

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 to perform ChIP Rat - Mesenteric arteries

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004 to perform ChIP Human - PANC-1

I have extracted RNA from brain tissue but my RNA concentrations are as low as 5ng/ml with my highest being around 80ng/ml. Will I be able to perform cDNA conversion with concentrations as low as these?

Get tips on using pET30 Ek/LIC-Colα3(IV) NC1 to perform Protein Expression Prokaryotic cells - E. coli Colα3(IV) NC1

Get tips on using DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #14793 to perform ChIP Anti-bodies FLAG

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?