siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells hsa-miR-542-3p

Get tips on using EpiTect ChIP OneDay Kit to perform ChIP Human - AGS

Get tips on using Re-ChIP-IT® to perform ChIP Human - LCL

Get tips on using STEMdiff™ Hematopoietic Kit to perform Stem cell Differentiation media Differentiation of Human iPSCs into microglia differentiation

Get tips on using ChIP Kit Magnetic - One Step (ab156907) to perform ChIP Human - MIA PaCa-2

Get tips on using Multiplex CRISPR/Cas9 Assembly System Kit to perform CRISPR Human - Activation hATCB

HeLa cells I used are passage 40. Are these cells good to perform Cell cycle analysis on?

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using PL_MmHspa5 to perform Protein Expression Eukaryotic cells - CHO Hspa5

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.