Get tips on using anti-Mucin 6, Clone: CLH5, Invitrogen™ to perform Immunohistochemistry Human - MUC-6
Get tips on using MUC5AC Antibody / Mucin-5AC [clone 45M1] (V2198) to perform Immunohistochemistry Human - Muc-5AC
Get tips on using Mucin 2 Antibody (F-2): sc-515032 to perform Immunohistochemistry Human - Muc-2
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human airway epithelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human chondrocytes - rheumatoid arthritis
A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.
A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.
Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Tissue - Human Adrenal glands
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Human Adrenal glands
Get tips on using pAC154-dual-dCas9VP160-sgExpression to perform CRISPR Human - Activation HIV-1 5′ LTR
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