Get tips on using CD115 (c-fms) Monoclonal Antibody (AFS98), Biotin, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD115
Get tips on using CD115 (c-fms) Monoclonal Antibody (AFS98), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD115
Get tips on using CD86 (B7-2) Monoclonal Antibody (GL1), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD86
Get tips on using CD8a Monoclonal Antibody (53-6.7), eFluor 450, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD8a
Get tips on using CD11b Monoclonal Antibody (M1/70), eFluor 450, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD11b
Get tips on using CD45 Monoclonal Antibody (30-F11), PE-Cyanine7, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD45
Get tips on using GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit to perform CRISPR Mouse - Deletion NIH 3T3 G3BP
Get tips on using GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit to perform CRISPR Mouse - Deletion NIH 3T3 FXR
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P) to perform Immunohistochemistry Mouse - TUBB3
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