DNA isolation / purification Cells Primary cells

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - Goblet cells

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Mel cells

Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - SAE cells

Get tips on using FlowCellect Autophagy LC3 antibody based kit to perform Autophagy assay cell type - K562 cells

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - K562 cells

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - K562 cells

Get tips on using LIVE/DEAD Fixable Violet Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - HEK 293

Get tips on using LIVE/DEAD Fixable Violet Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - mouse splenocytes

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.