DNA methylation profiling Gene specific profiling UMR-106

Get tips on using p-NOS3 Antibody (pT495.33): sc-136519 to perform Western blotting eNOS

Get tips on using p53 Antibody (DO-1): sc-126 to perform Western blotting p53

Get tips on using Anti-Beta Catenin CTNNB1 Antibody Picoband™ (Monoclonal, 1F6) to perform Immunohistochemistry Human - β-catenin

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Acridine Orange solution to perform Necrosis HCT 116

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - HCT-116

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - MLS-1765

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Necrosis HCT 116

Get tips on using GFP-CERTIFIED® Apoptosis/Necrosis detection kit to perform Necrosis HCT 116