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Get tips on using siGENOME Rat Acvr1c (245921) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - INS-1 Alk7/Acvr1c

Get tips on using siGENOME Human AP1G1 (164) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HeLa γ1-adaptin/AP1G1

Get tips on using Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649 to perform ChIP Anti-bodies H3K9-Ac

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Human fetal osteoblastic (hFOB) 1.19

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Peripheral blood mononuclear cells (PBMC)

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Peripheral blood mononuclear cells (PBMC)

Get tips on using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody to perform Autophagy assay cell type - HepG2

Get tips on using mRFP-green fluorescent protein (GFP)-LC3 adenoviral vector to perform Autophagy assay cell type - Macrophages

Get tips on using TUNEL Assay Kit - BrdU-Red to perform TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.