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Get tips on using pGEX-6P-1 Vector to perform Protein expression and purification Bacteria - Escherichia coli FleN

Products Sigma-Aldrich pGEX-6P-1 Vector

Get tips on using pQE-30 Xa Vector to perform Protein expression and purification Bacteria - Escherichia coli Siva1

Products Qiagen pQE-30 Xa Vector

Get tips on using miRCURY Exosome Serum/Plasma Kit to perform Purification of extracellular vesicles Exosomes - Seminal plasma

Products Qiagen miRCURY Exosome Serum/Plasma Kit

Get tips on using Ni-NTA Superflow (100 ml) to perform Protein tag Purification of His-tagged proteins

Products Qiagen Ni-NTA Superflow (100 ml)

Get tips on using Plasmid Mini to perform Plasmid Isolation Corynebacterium diphtheriae

Products A&A Biotechnology Plasmid Mini

Get tips on using NucleoBond® PC to perform Plasmid Isolation Enterobacteriaceae

Products Macherey Nagel NucleoBond® PC

Get tips on using QIAfilter Plasmid Kits to perform Plasmid Isolation Enterobacteriaceae

Products Qiagen QIAfilter Plasmid Kits

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat mesenchymal stem cells (rMSC)

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat dermal fibroblasts (rDF)

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

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