CRISPR Rat Deletion INS-1 832/13

Get tips on using FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme to perform TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells

Get tips on using Image-IT™ LIVE Green Reactive Oxygen Species Detection Kit, for microscopy to perform ROS assay cell type - MCF-7

Get tips on using Gyros IncSupplier Diversity Partner REXXIP HN BUFFER 25 ML PER VI DFS Item to perform Protein isolation Bacteria - Borrelia burgdorferi

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - immortalized PC-12

Get tips on using miRcute miRNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-157

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MCF 10A

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized A-172

Isolating RNA from tissues and paraffin embeded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the intigrity of RNA

Isolating RNA from tissues and paraffin embeded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the intigrity of RNA