siRNA / miRNA gene silencing Human MDA-MB-453

Get tips on using pONE-30A to perform Protein Expression Eukaryotic cells - S. frugiperda MBP

Get tips on using pONE-25A to perform Protein Expression Eukaryotic cells - P. pastoris MBP

Get tips on using pONE-10K to perform Protein Expression Prokaryotic cells - E. coli MBP

Get tips on using GenJet™ In Vitro DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines COS7

Get tips on using GenJet™ In Vitro DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines MCF-7

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Color-coded Prestained Protein Marker, High Range (43-315 kDa) #12949 to perform Protein Ladder Prestained

Get tips on using GeneJET RNA Purification Kit to perform AAA for reviews

Get tips on using GeneJET Plasmid Miniprep Kit to perform Plasmid Isolation Piscirickettsia salmonis

Get tips on using GeneJET Plasmid Miniprep Kit to perform Plasmid Isolation Lactobacillus brevis