DNA quantification Mouse

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - CAKI-1

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - HEK 293

Get tips on using Ribo-Zero® rRNA Removal Kit to perform RNA quantification Fuorimetric - human brain tissue

Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Mammalian cells - RAW264.7

Get tips on using Quant-iT™ RiboGreen™ RNA Assay Kit to perform RNA quantification Fuorimetric - HEK293

Get tips on using Quant-iT™ RiboGreen™ RNA Assay Kit to perform RNA quantification Fuorimetric - HeLa

Get tips on using SensiFAST™ Probe No-ROX One-Step Kit to perform RNA quantification qPCR - fibroblast

Get tips on using ON-TARGETplus Mouse Jun siRNA to perform siRNA / miRNA gene silencing Mouse - Embryonic stem cells Jun

Get tips on using ON-TARGETplus Mouse Gpam siRNA to perform siRNA / miRNA gene silencing Mouse - Embryonic stem cells Gpat1

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.