ELISA Mouse

Get tips on using Human CRP/C Reactive Protein PicoKine™ ELISA Kit to perform ELISA Human - C-Reactive Protein/CRP

Get tips on using HT 8-oxo-dG ELISA Kit II to perform DNA Damage Assay Capan-2

Get tips on using HT 8-oxo-dG ELISA Kit II to perform DNA Damage Assay MIA PaCa-2

Get tips on using Human RBP4/Retinol Binding Protein 4 PicoKine™ ELISA Kit to perform ELISA Human - RBP4

Get tips on using Human Chitinase 3-like 1/YKL-40 PicoKine™ ELISA Kit EK0974 to perform ELISA Human - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using FITC Mouse Anti-Mouse NK-1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1

Get tips on using APC Mouse Anti-Mouse NK-1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1

Get tips on using Alexa Fluor® 700 Mouse Anti-Mouse NK1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1

Get tips on using Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) to perform Flow cytometry Anti-bodies Mouse - CD16/CD32