Flow cytometry Anti-bodies

Get tips on using CD3e Monoclonal Antibody (145-2C11), Biotin, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD3

Get tips on using CD11c Monoclonal Antibody (N418), PE-Cyanine5.5, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD11c

Get tips on using CD11b Monoclonal Antibody (M1/70.15), PE-Texas Red to perform Flow cytometry Anti-bodies Mouse - CD11b

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using B7-H4 Monoclonal Antibody (188), PE, eBioscience™ to perform Flow cytometry Anti-bodies Human - B7-H4

Get tips on using Granzyme Monoclonal Antibody (NGZB), PE-Cyanine7, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - Granzyme B

Get tips on using Granzyme B Monoclonal Antibody (NGZB), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - Granzyme B

Get tips on using CD184 (CXCR4) Monoclonal Antibody (2B11), Biotin, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD184/CXCR4

Get tips on using F4/80 Monoclonal Antibody (BM8), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - F4/80