Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
Get tips on using Anti-LC3B antibody produced in rabbit to perform Western blot LC3B - Rabbit Human -NA-
Get tips on using MaXtract High Density (100 x 15 ml) to perform DNA isolation / purification Tissue - small intestine
Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.
Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.
Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media iPSCs or hESCs differentiation into Neuronal cells
Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media hESCs or iPSCs differentiation into ovarian follicle/granulosa cells
Get tips on using Gibco™Advanced DMEM/F-12 to perform Stem cell Differentiation media hPSCs or iPSCs differentiation into Lung progenitor cells
Get tips on using Gibco™DMEM/F-12, no glutamine to perform Stem cell Differentiation media hPSCs or iPSCs differentiation into Lung progenitor cells
Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell Differentiation media iPSCs or hESCs differentiation into Neuronal cells
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment